THE MRNA SECONDARY STRUCTURE’S FOLDING FOR CYPRINUS CARPIO CARPIO L., 1758 (CYPRINIDAE) INDIVIDUALS’ CONTROL REGION’S GENE HAPLOTYPES FROM MOVILENI AND IAŞI POPULATIONS
Lucian D. Gorgan
“Alexandru Ioan Cuza” University, Faculty of Biology, Department of Genetics, Iaşi, Romania
In present paper, the main purpose of the study was to establish the mRNA’s secondary structure for the control region molecular marker, for Cyprinus carpio carpio L. 1758 individuals, having the origin in two different populations (Movileni and Iaşi), as well as to compare those structures with similar models of the same gene, taken from the GenBank, for different species and subspecies of Cyprinus genera. Thus, regions from mtDNA were sequenced for 10 individuals of each analyzed population, based on a purified DNA matrix, using a method with phenol:chloroform:isoamyl alcohol (25:24:1). Those genes were subsequently amplified and sequenced using two complementary universal primers, obtaining both complementary chains, which ulterior formed a unique strand. MRNA’s secondary structure transcription was made using the GeneQuest module of LaserGene’s program (full trial version).